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| Roles |
| Sequences || BBa_K196002_sequence (Version 1)|
DescriptionCaulobacter crescentus is an aquatic, Gram-negative bacterium that divides asymmetrically and is able to synthetize a strong glue. This glue is mainly made of a polysaccharide. There are differents proteins needed to synthetize, export and attach it to the stalk of Caulobacter. To see the hole system, please see this page. In our project, we wanted this glue to be produced by Escherichia coli. As E. coli does have homologs for many of these proteins, but not for HfsG and HfsH, we decided to create a BB including only the genes coding for these two proteins. HfsG is a glycosyltransferase and HfsH is a carbohydrate esterase.
NotesAs many mutations were needed to make the part compatible with the standard 10, we decided to make it synthetized by GeneArt.
SourceThis sequence comes from Caulobacter crescentus.