BBa_K1964003

BBa_K1964003 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K1964003

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Steffen L??tke

Date created: 2016-10-13 11:00:00

Date modified: 2016-10-14 02:05:20

Codon optimized Cytochrome P450 for POH synthesis

Details

• Types: DnaRegion
• Roles: Coding; CDS
• Sequences: BBa_K1964003_sequence (Version 1)

Description

This part consists of the gene sequences for the three proteins that are needed for the synthesis of Perillyl alcohol from (-)-(S)-Limonene via the cytochrome P450 protein complex. The codon optimized sequence for Saccharomyces cerevisiae consists of the alkane hydroxylase, ferredoxin and ferredoxin reductase. The protein sequences are linked via T2A-sequences with slightly different DNA-sequences to avoid unwanted homologous recombination. The T2A-sequences allow the transcription of the three proteins from one promoter as the sequences mediate the cleavage of the peptide sequence.
The cytochrome P450 was used in the project of the Marburger iGEM-Team 2016 to produce the anti-cancer drug Perillyl alcohol in Saccharomyces cerevisiae from the (-)-(S)-Limonene that has been produced in the artificial endosymbiotic E. coli.

Notes

During the design the T2A-sequences for the correct cleavage of the peptide-sequences were added with slightly different DNA-sequences to avoid unwanted homologous recombination. Biobrick cutsites had to be removed from the codon optimized sequence for Saccharomyces cerevisiae before ordering the gene blocks from idtDNA.

Source

The genes were originally found in Mycobacterium sp. strain HXN-1500. The codon optimized genomic sequence used including the T2A-sequences have been synthesized via idtDNA.

Other Properties

• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K1964003/1

Member of these Collections

• iGEM Parts Registry