Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Nik Franko
Date created: 2016-10-16 11:00:00
Date modified: 2016-10-19 02:42:33
His:CRY:Myc:nLuc
| Types | DnaRegion |
| Roles | Coding CDS |
| Sequences | BBa_K1965007_sequence (Version 1) |
Description
This part is one of the two components of the dimeryzing system which is induced by blue light. We adapted this system for the reconstitution of split luciferase to create a blue-light sensor. To the C-terminus of CRY, N-terminus of split firefly luciferase was added. Upon induction with light and concequent dimerisation, the split luciferase is reconstituted and regains activity, which results in biolumiescence if luciferin is added.Notes
/Source
Arabidopsis thali and Photinus pyralis (Common eastern firefly)| Sequence Annotation | Location | Component / Role(s) |
| start His CRY2PHR 10GS linker Myc GSG linker nLuc stop | 1,3 4,21 22,1512 1513,1542 1543,1572 1573,1581 1582,3051 3052,3054 | start_codon feature/start feature/tag tag feature/cds CDS feature/misc sequence_feature feature/tag tag sequence_feature feature/misc CDS feature/cds stop_codon feature/stop |