Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Brandon Wilbanks
Date created: 2016-10-10 11:00:00
Date modified: 2016-10-29 08:42:33
lacPromoter-xylA-xylM
| Types | DnaRegion |
| Roles | CDS Coding |
| Sequences | BBa_K1966000_sequence (Version 1) |
Description
This part contains xylA and xylM from Pseudomonas putida under the control of a lac promoter. xylA and xylM are subunits of P. putida xylene monooxygenase (XO). XO converts a CH3 group of toluene and its derivatives into CH2OH. This is a basic part that is used in BBa_K1966002 with xylM (benzyl alcohol dehydrogenase, BADH) to generate aromatic aldehydes from toluene derivatives.Notes
xylA and xlyM were built together from synthesized DNA via Gibson assemblySource
Pseudomonas putida (TOL pathway, upper operon)| Sequence Annotation | Location | Component / Role(s) |
| lac promoter (part BBa_R0011) RBS (part BBa_B0034) xylA RBS (part BBa_B0034) xylM T1 Terminator (part BBa_B0010) | 1,55 56,67 68,1120 1121,1132 1133,2242 2243,2322 | feature/promoter promoter ribosome_entry_site feature/rbs sequence_feature feature/dna ribosome_entry_site feature/rbs sequence_feature feature/dna feature/misc sequence_feature |