Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Paolo Marangio
Date created: 2016-10-05 11:00:00
Date modified: 2016-10-17 11:51:36
Synechocystis Pcpc560 Phytobrick promoter
| Types | DnaRegion |
| Roles | promoter Regulatory |
| Sequences | BBa_K1968001_sequence (Version 1) |
Description
Pcpc560 stands for the 560bp region found upstream of the cpcB gene encoding the beta subunit of c-phycocyanin in Synechocystis. This region has been reported to act as a promoter that is able of producing heterologous proteins at a level up to 15% of total soluble protein in Synechocystis, which is comparable to levels achieved in E. coli (Zhou et al., 2014). In light of this, this genomic region has been termed as the Pcpc560 ???super-strong??? promoter.Zhou, J., Zhang, H., Meng, H., Zhu, Y., Bao, G., Zhang, Y., . . . Ma, Y. (2014). Discovery of a super-strong promoter enables efficient production of heterologous proteins in cyanobacteria. Sci Rep, 4, 4500. doi: 10.1038/srep04500
Notes
Nucleotide X at position X was mutated to a X in order to remove a BsaI site, which would have interfered with later assemblies.Source
The part was ordered as gblock through IDT and was subsequently cloned into PhytoBricks Universal Acceptor plasmids (BBa_P10500).| Sequence Annotation | Location | Component / Role(s) |
| Phytobrick Adapter Site A Promoter Pcpc560 Phytobrick Adapter Site C | 1,4 5,564 565,568 | sequence_feature feature/misc feature/promoter promoter sequence_feature feature/misc |