P-45

BBa_K1968007 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K1968007
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Ibrahim Al-Masoud
Date created: 2016-10-11 11:00:00
Date modified: 2016-10-19 03:02:30

P-45 Promoter Phytobrick



Types
DnaRegion

Roles
promoter

Regulatory

Sequences BBa_K1968007_sequence (Version 1)

Description

A widely used strong constitutive promoter from Corynebacterium (Patek et al., 1996) that works with related gram positive actinobacteria, such as Rhodococcus. We have this part in pUPD2, a MoClo-compatible level 0 vector with no illegal sites for MoClo assembly standard, GoldenBraid assembly standard and iGEM???s pairwise assembly standard.

Notes

The part was integrated in reverse and we advise the user to use the reverse complement sequence for downstream applications.

Source

This part was extracted through PCR from pSRKBB-empty vector (Addgene #59449) and was assembled into the PhytoBricks Universal Acceptor (BBa_P10500 or pUPD2). The sequence of this part was confirmed by Sanger Sequencing.

Sequence Annotation Location Component / Role(s)
Phytobrick Adapter Site A
Phytobrick Adapter SiteB
P-45 Promoter
1,4
151,154
5,150
feature/misc sequence_feature
sequence_feature feature/misc
promoter feature/promoter
igem#experience
None
 
igem#status
Planning
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K1968007/1