Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Alejandra Adriana Schiavon Osorio
Date created: 2016-10-12 11:00:00
Date modified: 2016-10-17 10:59:09
Phleomycin resistance cassette Phytobrick
| Types | DnaRegion |
| Roles | engineered_region Translational_Unit |
| Sequences | BBa_K1968015_sequence (Version 1) |
Description
Phleomycin is mutagenic by introducing double-strand breaks in DNA. The ble gene of Streptoalloteychus hindustanus, which confers resistance to this substance, is widely used as a selection marker for transformation. The device is designed for filamentous fungi and is controlled by the PgdaA, a constitutive promoter and the Tcyc terminator.Notes
The device has the A_E MoClo compatible phytobrick overhangs.Source
The sequence was provided by the Fungal Stock Genetic Center. It was PCR out from plasmid P444 from their collection.| Sequence Annotation | Location | Component / Role(s) |
| Phytobrick Adapter Site A PgdaA Ble Tcyc Phytobrick Adapter Site E | 1,4 5,680 681,1057 1142,1376 1396,1399 | feature/misc sequence_feature promoter feature/promoter CDS feature/cds feature/stop stop_codon feature/misc sequence_feature |