BBa_K1969002

BBa_K1969002 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K1969002
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Yijie Lai
Date created: 2016-10-04 11:00:00
Date modified: 2016-10-08 07:54:52

Homo sapiens adrenoceptor beta 2 (ADRB2) with 6 x His tag



Types
DnaRegion

Roles
CDS

Coding

Sequences BBa_K1969002_sequence (Version 1)

Description

Homo sapiens adrenoceptor beta 2 (ADRB2) is a G-protein coupled receptor (GPCR) activated by adrenaline that plays an important part in cardiovascular and pulmonary physiology, and is one of the most extensively characterized members of this large family of membrane proteins. While the natural protein doesn't contain any mark for us to visualize or quantify its expression, the 6 x his tag is for immunofluorescence and western blotting.

Notes

It may require site-specific mutagenesis to become assembly compatible.

Source

The sequence for Homo sapiens adrenoceptor beta 2 is from human genomic sequence, the sequence for 6 x his tag was added artificially.

Sequence Annotation Location Component / Role(s)
ADRB2
6 x His tag
ATG
TAA
1,1239
1240,1260
1,3
1258,1260
feature/protein CDS
feature/tag tag
start_codon feature/start
feature/stop stop_codon
igem#experience
None
 
igem#status
Planning
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K1969002/1