BBa_K197011

BBa_K197011 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K197011
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Gabriela Guzman Lopez Aguado
Date created: 2009-10-20 11:00:00
Date modified: 2015-05-08 01:11:18

{TshA}



Types
DnaRegion

Roles
Coding

CDS

Sequences BBa_K197011_sequence (Version 1)

Description

Autotransporter type: serine protease subfamily (because of the 7AA serine protease motif). Autotransporters are proteins that form a pore in the outermembrane and and pull their N terminus through this pore in order to expose it to the extracellular space. N-terminal fusions of proteins to this part may permit the cell surface display of the fused protein.

Notes

N/A

Source

 
PCR ca998/O09ig283 on M10088 (1575, EcoRI/BamHI)
Sub in pBca9523-Bca1144#5 (EcoRI/BamHI, 2472+1224, L)
Product is pBca9523-O09ig283 {}


Ca998 forward oligo
gtatcacgaggcagaatttcag
O09ig283 reverse oligo with stop codons removed for {}
gacggGGATCCgaatgaataacgaatattag


igem#experience
None
 
igem#sampleStatus
It's complicated
igem#status
Planning
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K197011/1