BBa_K197012

BBa_K197012 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K197012

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Gabriela Guzman Lopez Aguado

Date created: 2009-10-20 11:00:00

Date modified: 2015-05-08 01:11:18

{VirG(IcsA)}

Details

• Types: DnaRegion
• Roles: CDS; Coding
• Sequences: BBa_K197012_sequence (Version 1)

Description

Autotransporters are proteins that form a pore in the outermembrane and and pull their N terminus through this pore in order to expose it to the extracellular space. N-terminal fusions of proteins to this part may permit the cell surface display of the fused protein.

Notes

N/A

Source

PCR O09ig302/O09ig301 on S. Flexneri (490bp, gp = frag1)

PCR O09ig300/O09ig303 on S. Flexneri       (986bp, gp = frag2)



PCR O09ig302/O09ig303 on mixed frags    (1457bp, EcoRI/BamHI)

Sub in pBca9523-Bca1144#5           (EcoRI/BamHI, 2472+1224, L)

Product is pBca9523-B09ig302         {?VirG AtD?}



O09ig302    Forward PCR of {?VirG AtD?} (B09ig302)

AAGTGGAATTCATGAGATCTTCGTCAAATGTCGGTCAGAC

O09ig301    Reverse internal oligo for {?VirG AtD?} (B09ig302)

CTGCCAGCCTCAGGGCGCC

O09ig300    Forward internal oligo for {?VirG AtD?} (B09ig302)

GGCGCCCTGAGGCTGGCAG

O09ig303    Reverse PCR of {?VirG AtD?} (B09ig302)

GTTAGGGATCCGAAGGTATATTTCACACCC

Other Properties

• igem#experience: None
• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K197012/1

Member of these Collections

• iGEM Parts Registry