BBa_K197014

BBa_K197014 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K197014

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Gabriela Guzman Lopez Aguado

Date created: 2009-10-20 11:00:00

Date modified: 2015-05-08 01:11:18

{AIDA-I}

Details

• Types: DnaRegion
• Roles: CDS; Coding
• Sequences: BBa_K197014_sequence (Version 1)

Description

Features: identified to be similar to IgA1, the first autotransporter used for surface display. Occurs naturally in the host organism, E. coli, and is a robust tool for surface display. Autotransporters are proteins that form a pore in the outermembrane and and pull their N terminus through this pore in order to expose it to the extracellular space. N-terminal fusions of proteins to this part may permit the cell surface display of the fused protein.

Notes

N/A

Source

PCR O09ig310/O09ig309 on EHEC (1184bp, gp = frag1)

PCR O09ig308/O09ig311 on EHEC (287bp, gp = frag2)



PCR O09ig310/O09ig311 on mixed frags    (1450bp, EcoRI/BamHI)

Sub in pBca9523-Bca1144#5           (EcoRI/BamHI, 2472+1224, L)

Product is pBca9523-B09ig310         {?AIDA-1 AtD?}



O09ig310    Forward PCR of {?AIDA-1 AtD?} (B09ig310)

AAGTGGAATTCATGAGATCTGCTGGCAATGTGTTAGTGGTG

O09ig309    Reverse internal oligo for {?AIDA-1 AtD?} (B09ig310)

ATGGTGGCTCTTGAGCCAGGTTTT

O09ig308    Forward internal oligo for {?AIDA-1 AtD?} (B09ig310)

AAAACCTGGCTCAAGAGCCACCAT

O09ig311    Reverse PCR of {?AIDA-1 AtD?} (B09ig310)

GTTAGGGATCCGAATTGCCACTTAATGCCAAC

Other Properties

• igem#experience: None
• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K197014/1

Member of these Collections

• iGEM Parts Registry