BBa_K197017

BBa_K197017 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K197017

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Gabriela Guzman Lopez Aguado

Date created: 2009-10-20 11:00:00

Date modified: 2015-05-08 01:11:18

CPG_L6 (circularly permuted OmpG)

Details

• Types: DnaRegion
• Roles: Coding; CDS
• Sequences: BBa_K197017_sequence (Version 1)

Description

Features: protein is circularly permuted with its backbone opening in loop 6, allowing both the N- and C- termini to be present in the extracellular space. Autotransporters are proteins that form a pore in the outermembrane and and pull their N terminus through this pore in order to expose it to the extracellular space. N-terminal fusions of proteins to this part may permit the cell surface display of the fused protein.

Notes

N/A

Source

PCR ca998/O09ig279 on M10011		  		(873, EcoRI/BamHI)

Sub in pBca9523-Bca1144#5           	(EcoRI/BamHI, 2472+1224, L)

Product is pBca9523-B09cpgl6  				{}



ca998	Forward Oligo 

CCATAGAATTCATGAGATCTTGGACCCAGATGTCTTCTGGCACTC

O09cpgl6	Reverse Oligo for the removal of stop codon from {}

CGTTAGGATCCctgccagtcccagttactccagcg	

Other Properties

• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K197017/1

Member of these Collections

• iGEM Parts Registry