Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Shuli Liang
Date created: 2016-10-12 11:00:00
Date modified: 2016-10-13 10:45:31
dszBCAD with T7 promoter
| Types | DnaRegion |
| Roles | CDS Coding |
| Sequences | BBa_K1972007_sequence (Version 1) |
Description
We constructed dszBCAD genes with T7 promoter and choose E.coli BL21 as our host cell. When IPTG is added to our system, DszABCD enzymes will be successful expressed and these four enzymes convert dibenzothiophene (DBT) undergoes through three successive oxidation steps and one a hydrolytic step to 2-hydroxybiphenyl (2HBP) by the 4s pathway.Each single enzyme gene was synthesized by Generay and we constructed them together and suquencing comformed. the sequences can be found in NCBI (GenBank Accession number L37363.1)
Notes
No.Source
Each single enzyme gene was synthesized by Generay and we constructed them together and suquencing comformed. the sequences can be found in NCBI (GenBank Accession number L37363.1)| Sequence Annotation | Location | Component / Role(s) |
| T7lac promoter RBS dszB RBS dszC RBS dszA RBS dszD double teminator | 1,44 45,63 64,1161 1170,1189 1190,2443 2451,2472 2473,3834 3843,3862 3863,4441 4442,4537 | feature/promoter promoter feature/rbs ribosome_entry_site feature/cds CDS ribosome_entry_site feature/rbs feature/cds CDS feature/rbs ribosome_entry_site feature/cds CDS feature/rbs ribosome_entry_site feature/cds CDS sequence_feature feature/misc |