Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Shuli Liang
Date created: 2016-10-13 11:00:00
Date modified: 2016-10-14 07:47:04
dszBCAD with Lac promoter
| Types | DnaRegion |
| Roles | CDS Coding |
| Sequences | BBa_K1972009_sequence (Version 1) |
Description
We constructed dszBCAD genes with T7 promoter and choose E.coli BL21 as our host cell. When IPTG is added to our system, DszABCD enzymes will be successful expressed and these four enzymes convert dibenzothiophene (DBT) undergoes through three successive oxidation steps and one a hydrolytic step to 2-hydroxybiphenyl (2HBP) by the 4s pathway.Each single enzyme gene was synthesized by Generay and we constructed them together and suquencing comformed. the sequences can be found in NCBI (GenBank Accession number L37363.1)
Notes
Not yet.Source
Each single enzyme gene was synthesized by Generay and we constructed them together and suquencing comformed. the sequences can be found in NCBI (GenBank Accession number L37363.1)| Sequence Annotation | Location | Component / Role(s) |
| LacUV5 promoter dszB dszC dszA dszD double teminator LacI promoter LacI double teminator | 1,43 63,1160 1189,2442 2472,3833 3862,4440 4441,4569 4570,4647 4771,5730 5731,5859 | feature/promoter promoter feature/cds CDS feature/cds CDS CDS feature/cds CDS feature/cds feature/misc sequence_feature promoter feature/promoter feature/cds CDS feature/misc sequence_feature |