BBa_K1972011

BBa_K1972011 Version 1

Component

This part has been discontinued.

Source:
http://parts.igem.org/Part:BBa_K1972011
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Shuli Liang
Date created: 2016-09-15 11:00:00
Date modified: 2016-10-14 07:10:32

T7 RNA polymerase induced by IPTG



Types
DnaRegion

Roles
engineered_region

Composite

Sequences BBa_K1972011_sequence (Version 1)

Description

T7 RNA polymerase will not be expressed without IPTG. When IPTG is added to 0.3 mM, T7 RNA polymerase will be expressed and if there is protein gene with T7 promoter, it will be greatly expressed by the host cell.

Notes

No.

Source

We use PCR to get this part from E.coli BL21 and use BBa_B0015 as a teminator.

Access Instance Definition
public
BBa_B0015
BBa_B0015
Sequence Annotation Location Component / Role(s)
BBa_B0015
1,129
BBa_B0015
igem#experience
None
 
igem#status
Deleted
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K1972011/1