Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Laura Claret Fern??ndez
Date created: 2016-10-08 11:00:00
Date modified: 2016-10-09 11:55:05
Pm (2)
| Types | DnaRegion |
| Roles | Regulatory promoter |
| Sequences | BBa_K1973014_sequence (Version 1) |
Description
Pm2 is one variant of the promoter for the xylS/Pm expression system. It was obtained by site-directed mutagenesis PCR from the Pseudomonas putida plasmid pMPO52 in order to remove illegal restriction sites. The expression of this promoter is activated by XylS or XylS2 in the presence of benzoate and salicylate, respectively.Notes
It had to be site-directed mutagenized in position 65 in order to remove a PstI restriction site.Source
It was obtained by PCR from a plasmid containing its sequence.| Sequence Annotation | Location | Component / Role(s) |
| Pm (2) A -> G mutation | 1,388 65,65 | feature/promoter promoter sequence_alteration feature/mutation |