Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Laura Claret Fern??ndez
Date created: 2016-10-08 11:00:00
Date modified: 2016-10-13 10:43:08
pleD*
| Types | DnaRegion |
| Roles | CDS Coding |
| Sequences | BBa_K1973016_sequence (Version 1) |
Description
pleD* BBa_K1973016 Codifies the PleD* protein from Caulobacter crescentus, which is a diguanilate cyclase that induces the biofilm formation by increasing c-di-GMP (cyclic diguanilate monophosphate) levels. c-diGMP regulates both the formation and dispersion of the biofilm, depending on the quantity of it that is present inside the cell. High levels of c-diGMP will increase the biofilm formation, and low levels of it will induce its dispersion. To manipulate this, we will make use of a phosphodiesterase, that will destroy the c-diGMP, and a diguanilate cyclase, that will produce it, YhjH and PleD* respectively.Contains a constitutive mutation in the original sequence (see BBa_K2012002) that improves the enzymatic activity by knocking out the regulation system.