BBa_K1973033

BBa_K1973033 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K1973033
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Laura Claret Fern??ndez
Date created: 2016-10-08 11:00:00
Date modified: 2016-10-09 11:17:26

nahR-Psal (inducible promoter)



    • Details

    Types
    DnaRegion

    Roles
    promoter

    Regulatory

    Sequences BBa_K1973033_sequence (Version 1)

    Description

    nahR (Pseudomonas putida, see BBa_K1031610) is expressed under the constitutive Pr promoter. It encodes a regulatory protein that activates the Psal promoter (see BBa_J61051) in the presence of salicylate.

    Notes

    We had included the RBS BBa_B0034 in the PCR primers, as well as the scar it should have appeared in the cloning of the DNA piece.

    Source

    It was obtained by PCR from a plasmid containing this sequence. The PCR primers added the prefix and suffix needed.

    Sequence Annotation Location Component / Role(s)
    Psal
    nahR
    		901,1039
    1,900
    		feature/promoter promoter
    CDS feature/cds

    igem#status
    Planning
     
    synbiohub#ownedBy
    user/james
     
    synbiohub#ownedBy
    user/myers
     
    synbiohub#topLevel
    BBa_K1973033/1
     

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