| Types | DnaRegion
|
| Roles | CDS
Coding
|
| Sequences | BBa_K1973034_sequence (Version 1)
|
Description
yhjH encodes the YhjH protein, a c-diGMP (cyclic diguanilate monophosphate) phosphodiesterase. c-diGMP regulates both the formation and dispersion of the biofilm, depending on the quantity of it that is present inside the cell. High levels of c-diGMP will increase the biofilm formation, and low levels of it will induce its dispersion. To manipulate this, we will make use of a phosphodiesterase, that will destroy the c-diGMP, and a diguanilate cyclase, that will produce it, YhjH and PleD* respectively.
Notes
We have inlcuded the sequence of RBS BBa_B0034 by means of the PCR primers used for the amplification, as well as the scar it should have been in case of the clonation of this part.
Source
It was obtained by PCR from the genome of E.coli DH5 alpha.
