BBa_K1973037

BBa_K1973037 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K1973037
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Alvaro Escobar Doncel
Date created: 2016-10-10 11:00:00
Date modified: 2016-10-12 05:15:26

nasR



Types
DnaRegion

Roles
Coding

CDS

Sequences BBa_K1973037_sequence (Version 1)

Description

NasR is RNA-binding protein that inhibits transcription termination dependent on the nasF terminator (see BBa_K1973001).

Notes

We have included the sequence of RBS BBa_B0034 and the scar it should have been in case of the clonning of this part. When we disigned the sequence to synthesize, we change the nucleotide in position 534 in order to remove a PstI restriction site.

Source

It was obtained by synthesis.

Sequence Annotation Location Component / Role(s)
B0034
scar
G -> A
nasR
1,12
13,18
159,159
19,1212
ribosome_entry_site feature/rbs
feature/scar
feature/mutation sequence_alteration
feature/cds CDS
igem#experience
None
 
igem#status
Planning
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K1973037/1