Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: YU-CHUN WU
Date created: 2016-10-12 11:00:00
Date modified: 2017-03-28 10:00:52
T7 Promoter+RBS+OAIP+linker+6X His-Tag
| Types | DnaRegion |
| Roles | engineered_region Composite |
| Sequences | BBa_K1974013_sequence (Version 1) |
Description
By ligating the IPTG induced promoter T7 (BBa_ I712074), strong ribosome binding site (BBa_B0034), OAIP, linker, and the 6xHistag (BBa_ K1223006), we are able to express OAIP, the toxin by IPTG induction.This year we create a revolutionary system that integrates biological pesticides, automatic detector, sprinkler, and IoT. We made a database that contains most of the spider toxins and selected the target toxins by programming. Orally Active Insecticidal Peptide is coded for the venom of a spider, Selenotypus plumipes. It is under the control of the strong T7 promoter. A 6xHistag is added for further protein purification
Notes
It is under the control of the strong T7 promoter. A 6xHistag is added for further protein purification.Source
Artificial Synthesis| Access | Instance | Definition |
| public public public | BBa_I712074 BBa_B0034 BBa_K1223006 | BBa_I712074 BBa_B0034 BBa_K1223006 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_I712074 BBa_B0034 BBa_K1223006 | 1,46 55,66 75,101 | BBa_I712074 BBa_B0034 BBa_K1223006 |