BBa_K1979010

BBa_K1979010 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K1979010
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: MA Xinyi
Date created: 2016-08-24 11:00:00
Date modified: 2016-08-25 02:23:47

Promoter (BBa_J23101)+PBP5+T7 terminator



    • Details

    Types
    DnaRegion

    Roles
    Device

    engineered_region

    Sequences BBa_K1979010_sequence (Version 1)

    Description

    This device codes for Penicillin binding protein 5. The coding sequence is tagged by 6??his and T7, and it is controlled by a constitutive promoter BBa_J23101 and a T7 terminator.

    Notes

    We added the histidine tag for affinity purification to harvest the proteins.

    Source

    The PBP5 and terminator sequences are retrieved form GenBank, and the promoter sequence comes from the part BBa_J23101.

    Access Instance Definition
    public
    public
    		BBa_J23101
    BBa_K1979000
    		BBa_J23101
    BBa_K1979000

    Sequence Annotation Location Component / Role(s)
    BBa_J23101
    BBa_K1979000
    		1,35
    44,1252
    		BBa_J23101
    BBa_K1979000

    igem#status
    Planning
     
    synbiohub#ownedBy
    user/james
     
    synbiohub#ownedBy
    user/myers
     
    synbiohub#topLevel
    BBa_K1979010/1
     

    Attachments

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