MymT

BBa_K1980002 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K1980002

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Sam Garforth

Date created: 2016-10-10 11:00:00

Date modified: 2016-10-23 03:09:02

MymT

Details

• Types: DnaRegion
• Roles: Coding; CDS
• Sequences: BBa_K1980002_sequence (Version 1)

Description

MymT is a small prokaryotic copper metallothein discovered in Mycobacterium tuberculosis. It is believed that the protein may help the bacterium survive copper toxicity, though deleting the gene had no effect on pathogenicity in mice. It can bind up to 7 copper ions but has a preference for 4-6. Our version has a C terminal hexahistidine tag for purification and has been codon optimised for E. coli.

Notes

This protein has a C terminal hexahistidine tag for purification and has been codon optimised for E. coli.

Source

Mycobacterium tuberculosis

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
MymT	1,159	CDS, feature/cds
Hexahistidine	160,177	feature/tag, tag
Double stop	178,183	feature/stop, stop_codon

Other Properties

• igem#experience: Issues
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K1980002/1

Member of these Collections

• //binding/metal
• iGEM Parts Registry