Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Zexu Li
Date created: 2016-09-10 11:00:00
Date modified: 2016-10-09 12:15:31
Prokaryotic Cryptochrome 2 (CRY2) ( a blue light stimulated photoreceptor)
| Types | DnaRegion |
| Roles | Coding CDS |
| Sequences | BBa_K1982002_sequence (Version 1) |
Description
Cryptochrome 2 (CRY2) is a blue light stimulated photoreceptor, when exposed to blue light, it would interact withthe N-terminal fragment of CIB1 (CIBN) . This part is full-length CRY2 CDS.Optogenetic systems enable precise spatial and temporal control of cell behavior. A light-activated CRISPR/Cas9 effector (LACE) system that induces transcription of endogenous genes in the presence of blue light.This was accomplished by fusing the light-inducible heterodimerizing proteins CRY2 and CIB1 to a transactivation domain and the catalytically inactive tCas9, respectively. The versatile LACE system can be easily directed to new DNA sequences for the dynamic regulation of endogenous genes.
Notes
mutant EcoR 1 : TGTTGGGAATTc--->TGTTGGGAATTgSource
The light-inducible heterodimerizering proteins CRY2 and CIB1 are both from Arabidopsis thaliana.| Sequence Annotation | Location | Component / Role(s) |
| ATG Prokaryotic rbs | 19,21 1,18 | start_codon feature/start feature/rbs ribosome_entry_site |