BBa_K1983002

BBa_K1983002 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K1983002
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Vykintas Jauni??kis
Date created: 2016-09-13 11:00:00
Date modified: 2016-10-16 04:36:39

gp45 phenylalanine mutant M6 with C-terminal 6XHis-Tag



    • Details

    Types
    DnaRegion

    Roles
    CDS

    Coding

    Sequences BBa_K1983002_sequence (Version 1)

    Description

    T4 phage clamp protein gp45 with 6 stable mutations to phenylalanine.

    Notes

    Designed including NdeI restriction site for efficient cloning into expression vectors, XhoI site for cutting between the protein and 6XHis-Tag.

    Source

    T4 phage

    Sequence Annotation Location Component / Role(s)
    gp45M6
    C-terminal 6XHis-Tag
    Double translation stop codon
    		4,687
    688,711
    712,714
    		CDS feature/protein
    tag feature/tag
    feature/stop stop_codon

    igem#status
    Planning
     
    synbiohub#ownedBy
    user/james
     
    synbiohub#ownedBy
    user/myers
     
    synbiohub#topLevel
    BBa_K1983002/1
     

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