BBa_K1983003

BBa_K1983003 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K1983003
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Vykintas Jauni??kis
Date created: 2016-09-14 11:00:00
Date modified: 2016-10-16 05:04:04

gp45 phenylalanine mutant M11 with C-terminal 6XHis-Tag



Types
DnaRegion

Roles
CDS

Coding

Sequences BBa_K1983003_sequence (Version 1)

Description

T4 phage clamp protein gp45 with 6 stable mutations to phenylalanine.

Notes

Designed including NdeI restriction site for efficient cloning into expression vectors, XhoI site for cutting between the protein and 6XHis-Tag.

Source

T4 phage

Sequence Annotation Location Component / Role(s)
gp45M11
C-terminal 6XHis-Tag
Double translation stop codon
4,687
688,711
712,714
CDS feature/protein
feature/tag tag
feature/stop stop_codon
igem#status
Planning
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K1983003/1