BBa_K1985003

BBa_K1985003 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K1985003
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Jasmine Cornish
Date created: 2016-10-13 11:00:00
Date modified: 2016-10-19 02:07:09

mamP, his-tagged, signal sequence cleaved



Types
DnaRegion

Roles
Coding

CDS

Sequences BBa_K1985003_sequence (Version 1)

Description

Usage: The mamX gene produces a protein that can be used to transfer electrons to iron molecules. It can be used in combination with other mam genes to form an electron transport complex (reference) that should form magnetic magnetite crystals when the cell is exposed to into in solution. It was to assemble a construct of MamO, P, T and X in pSB1A3 for in vivo expression.
Biology:
MamX is a proposed cytochrome protein (reference) due to the presence of a heme binding motif within its sequence. It forms a complex with other Mam proteins on the membrane of the native species, Magnetospirillum gryphiswaldense. Together they promote magnetite crystal maturation within an organelle called the magnetosome.

Notes

Codon Optimised

Source

The part comes from Magnetospirillum gryphiswaldense, sourced from Rokas Juodeikis in Professor Martin Warren's lab at the University of Kent.

Sequence Annotation Location Component / Role(s)
SecS-sol-mamP
15,851
CDS feature/cds
igem#experience
None
 
igem#status
Planning
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K1985003/1