Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Bowman Clark
Date created: 2016-10-13 11:00:00
Date modified: 2016-10-14 06:12:31
his-tagged PPGK ATPi homologue w/ Anderson Promoter J23106
| Types | DnaRegion |
| Roles | CDS Coding |
| Sequences | BBa_K1988011_sequence (Version 1) |
Description
PPGK
Overview
Name:Polyphosphate Dependent Glucokinase
Function:
PPGK catalyzes the first step in glycolysis, and therefore the presence of this enzyme suggests the importance of polyphosphate to metabolism in M. phosphovorus. [1]. Still, polyphosphate is not the only source of phosphorus in glycolysis, as evidenced by an ATP-only phosphofructokinase, which catalyzes the phosphorylation of D-fructose 6-phosphate [2].
In Microlunatus phosphovorus: M. phosphovorus is the first organism reported to have two homologs of PPGK. Microlunatus phosphovorus is unique because, whereas other Actinobacteria species have PPGKs that utilize both ATP and polyphosphate as a phosphorus donor, M. phosphovorus has a PPGK that has been found to only use polyphosphate as a phosphorus donor. The second PPGK homolog has yet to be characterized, so whether or not that PPGK utilizes both ATP and polyphosphate or just polyphosphate is unknown. [1]
Location: Cytoplasm
While the exact location of PPGK in the cytoplasm of Microlunatus phosphovorus is unknown, PPGK is located in the volutin granules of the Actinobacteria Corynebacterium glutamicum[3].The PPGKs in M. phosphovorus could possibly be located in the volutin granules as well.
Cofactors and Modifications: There are currently no known cofactors or modifications of the PPGK homolog and ATP independent PPGK in M. phosphovorus.
Images: The three-dimensional structure of all proteins were predicted using the I-TASSER software [4]. All images shown are the models with the highest confidence, which is quantitatively measured with a C-score that typically falls within the range [-5,2], in which 2 represents the highest level of confidence, and -5 represents the lowest level of confidence.
PPGK ATP Independent (ATPI)
Locus tag: MLP_05430
Image
C-score: 0.91
Gene length: 801 base pairs
Protein size: 28.1 kDa
Amino acid sequence: MTDTPPVAAP GRSVLGIDIG GSGIKGAPVD LATGLFAAER LRIDTPAKST PANVAKVVAE IVDHFKAEVG DGPIGITIPA VVTHGQTRSA ANIDHSWIDA EAEQIFEDVL QRDIYLMNDA DAAGIAEVHY GAAKGHPGLV IVTTLGTGIG SAMIHRGVLI PNSELGHLEI DGLDAETNAA SSAKERNDWS YSEWAPKLQR YYERLEALFW PDLIVVGGGV SKKAHKFLPK LKLKSQIIPA QLLNTAGIVG AAWLAADRLV HPDPMG [5]
In Microlunatus phosphovorus: PPGK ATPI can only use polyphosphate, and not ATP, as a phosphate donor. [1]
pH Range Optimal pH: 5.5
Temperature Range Optimal temperature: 30??C [2]
PPGK homolog
Locus tag: MLP_26610
Image
C-score: 1.25
Gene length: 762 base pairs
Protein size:
Amino acid sequence: MNETANIALG IDIGGTGIKG ALVDLETGAL VSDRFRLDTP RPALPAAVAD TVVAVAAHFD FAGPVGVAFP GVVLDGVVHT AANLHPDWIG ASLAELVGSR LSGPSVFLND ADAAGLAEAR FGAAKGVSGV VLLVTLGTGI GTAMISDGQL VPNSEFGHLE LDGLDAETYA AASARKRNNH TWEEWAGHAE HYLKYLEGLV WPKLFVLGGG ITKNPELWLH YLKPRTPIVL ATNINNAGII GAAAAAAQTQ QAG [6]
In Microlunatus phosphovorus: This PPGK homolog has yet to be characterized, so it is unknown whether or not this PPGK homolog can utilize ATP as a phosphate donor. [1]
pH Range The PPGK homolog in M. phosphovorus has yet to be characterized, and so there is no data for the pH range in which it functions.
Temperature Range The PPGK homolog in M. phosphovorus has yet to be characterized, and so there is no data for the temperature range in which it functions.
References
[1] A. Kawakoshi, H. Nakazawa, J. Fukada, M. Sasagawa, Y. Katano, S. Nakamura, A. Hosoyama, H. Sasaki, N. Ichikawa, S. Hanada, Y. Kamagata, K. Nakamura, S. Yamazaki and N. Fujita, "Deciphering the Genome of Polyphosphate Accumulating Actinobacterium Microlunatus phosphovorus", DNA Research, vol. 19, no. 5, pp. 383-394, 2012.
[2] S. Tanaka, S. Lee, K. Hamaoka, J. Kato, N. Takiguchi, K. Nakamura, H. Ohtake and A. Kuroda, "Strictly Polyphosphate-Dependent Glucokinase in a Polyphosphate-Accumulating Bacterium, Microlunatus phosphovorus", Journal of Bacteriology, vol. 185, no. 18, pp. 5654-5656, 2003.
[3] S. Pallerla, S. Knebel, T. Polen, P. Klauth, J. Hollender, V. Wendisch and S. Schoberth, "Formation of volutin granules in Corynebacterium glutamicum", FEMS Microbiology Letters, vol. 243, no. 1, pp. 133-140, 2005.
[4] Yang, R. Yan, A. Roy, D. Xu, J. Poisson and Y. Zhang, "The I-TASSER Suite: protein structure and function prediction", Nature Methods, vol. 12, no. 1, pp. 7-8, 2014.
[5] "ppgK - Polyphosphate-dependent glucokinase - Microlunatus phosphovorus - ppgK gene & protein", Uniprot, 2016. [Online]. Available: http://www.uniprot.org/uniprot/Q76LT2. [Accessed: 22- Jul- 2016].
[6] "ppgK - Polyphosphate-dependent glucokinase - Microlunatus phosphovorus (strain ATCC 700054 / DSM 10555 / JCM 9379 / NBRC 101784 / NCIMB 13414 / VKM Ac-1990 / NM-1) - ppgK gene & protein", Uniprot, 2016. [Online]. Available: http://www.uniprot.org/uniprot/F5XI06. [Accessed: 22- Jul- 2016].
Notes
Codon optimized for E. coli K-12 using IDT Codon OptimizerSource
Genetic sequence from 'Deciphering the Genome of Polyphosphate Accumulating Actinobacterium Microlunatus Phosphovorus' by Kawakoshi, et. al.| Access | Instance | Definition |
| public public public public | BBa_J23106 BBa_J34803 BBa_K1988010 BBa_K844000 | BBa_J23106 BBa_J34803 BBa_K1988010 BBa_K844000 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_J23106 BBa_J34803 BBa_K1988010 BBa_K844000 | 1,35 44,56 63,860 869,904 | BBa_J23106 BBa_J34803 BBa_K1988010 BBa_K844000 |