BBa_K1989003

BBa_K1989003 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K1989003

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Shijun Zhao

Date created: 2016-10-11 11:00:00

Date modified: 2016-10-12 12:01:18

Triple-spytag followed by amilGFP

Details

• Types: DnaRegion
• Roles: Coding; CDS
• Sequences: BBa_K1989003_sequence (Version 1)

Description

Triple-spytag followed by amilGFP

Notes

The length of the linker between spycatcher may affect the physical and chemical property of hydrogel.

Source

Inspiring from the self-catalysis of isopeptide bond between Lys and Asp in Streptococcus pyogenes fibronectin-binding protein FbaB, researchers split the catalytic domain and obtain two peptide called Spytag(the short one) and Spycatcher(the long one) which are able to form isopeptide bond with the other without any assistant.

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
his-tag	30,68	feature/tag, tag
triple spytag	69,317	CDS, feature/protein
amilGFP	405,1097	CDS, feature/protein

Other Properties

• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K1989003/1

Member of these Collections

• iGEM Parts Registry