BBa_K1989004

BBa_K1989004 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K1989004

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Shijun Zhao

Date created: 2016-10-11 11:00:00

Date modified: 2016-10-12 12:04:33

Triple-spytag followed by mRFP

Details

• Types: DnaRegion
• Roles: CDS; Coding
• Sequences: BBa_K1989004_sequence (Version 1)

Description

Triple-spytag followed by mRFP

Notes

Inspiring from the self-catalysis of isopeptide bond between Lys and Asp in Streptococcus pyogenes fibronectin-binding protein FbaB, researchers split the catalytic domain and obtain two peptide called Spytag(the short one) and Spycatcher(the long one) which are able to form isopeptide bond with the other without any assistant.

Source

The length of the linker between spycatcher may affect the physical and chemical property of hydrogel.

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
his-tag	30,68	tag, feature/tag
triple spytag	69,317	CDS, feature/protein
mRFP	405,1079	feature/protein, CDS

Other Properties

• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K1989004/1

Member of these Collections

• iGEM Parts Registry