Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Yiming Dong
Date created: 2016-10-10 11:00:00
Date modified: 2016-10-11 09:54:36
Triplespytag with mRFP, His-tag , FlAsH-tag and signal peptide PhoA
| Types | DnaRegion |
| Roles | Coding CDS |
| Sequences | BBa_K1989037_sequence (Version 1) |
Description
The fused protein PhoA-Histag-3A-mRFP-FlAsHtag possess both isopeptide bond forming function, uranyl-binding ability and secretion. Thus, using PhoA-Histag-3A-mRFP-FlAsHtag as a part of hydrogel formation, we can obtain our multifunctional biomaterial.Notes
The length of the linker between spytag may affect the physical and chemical property of hydrogel. As several attributes of our target proteins, especially their folding state in cells, we were unable to rationally design or choose a signal peptide for each. Nevertheless, we could build a Signal Peptide Repertory to screen for the best SP candidate. Our ultimate goal was to select the most appropriate ones from a huge amount of signal peptides.Source
Inspiring from the self-catalysis of isopeptide bond between Lys and Asp in Streptococcus pyogenes fibronectin-binding protein FbaB, researchers split the catalytic domain and obtain two peptide called Spytag(the short one) and Spycatcher(the long one) which are able to form isopeptide bond with the other without any assistant.| Sequence Annotation | Location | Component / Role(s) |
| Signal peptide PhoA His-tag Spytag-Spytag-Spytag-mRFP FlAsH-tag | 24,90 91,129 130,1143 1144,1186 | CDS feature/protein feature/tag tag CDS feature/protein tag feature/tag |