BBa_K1989041

BBa_K1989041 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K1989041
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Yiming Dong
Date created: 2016-10-11 11:00:00
Date modified: 2016-10-11 11:08:02

Triplespytag with mRFP, FlAsH-tag and signal peptide NprE



Types
DnaRegion

Roles
CDS

Coding

Sequences BBa_K1989041_sequence (Version 1)

Description

The fused protein NprE-3A-mRFP-FlAsHtag possess both isopeptide bond forming function, uranyl-binding ability and secretion. Thus, using NprE-3A-mRFP-FlAsHtag as a part of hydrogel formation, we can obtain our multifunctional biomaterial.

Notes

The length of the linker between spytag may affect the physical and chemical property of hydrogel. As several attributes of our target proteins, especially their folding state in cells, we were unable to rationally design or choose a signal peptide for each. Nevertheless, we could build a Signal Peptide Repertory to screen for the best SP candidate. Our ultimate goal was to select the most appropriate ones from a huge amount of signal peptides.

Source

Inspiring from the self-catalysis of isopeptide bond between Lys and Asp in Streptococcus pyogenes fibronectin-binding protein FbaB, researchers split the catalytic domain and obtain two peptide called Spytag(the short one) and Spycatcher(the long one) which are able to form isopeptide bond with the other without any assistant.

Sequence Annotation Location Component / Role(s)
Signal peptide NprE
Spytag-Spytag-Spytag-mRFP
FlAsH-tag
60,140
144,1157
1161,1199
CDS feature/protein
feature/protein CDS
tag feature/tag
igem#status
Planning
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K1989041/1