BBa_K199031

BBa_K199031 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K199031
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Olivia Ho-Shing
Date created: 2009-07-12 11:00:00
Date modified: 2015-05-08 01:11:18

pBad-CCAUC Suppressor tRNA (9-bp Anticodon)



Types
DnaRegion

Roles
mature_transcript_region

RNA

Sequences BBa_K199031_sequence (Version 1)

Description

Causes low expression of a suppressor tRNA that suppresses a 5-bp codon CCAUC, Parts K199007 and K199008.

Notes

We used the pBad promoter to transcribe lower levels of tRNA in a high copy plasmid, pSB1A2.

Source

The pBad promoter is from the BioBrick Parts registry. Suppressor tRNA was made by de novo synthesis from single-stranded oligos. Based on the paper by Anderson et al. 2002.

igem#experience
Fails
 
igem#sampleStatus
It's complicated
igem#status
Planning
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K199031/1