Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Leland Taylor
Date created: 2009-07-13 11:00:00
Date modified: 2015-05-08 01:11:19
pBad-CCCUC Suppressor tRNA
| Types | DnaRegion |
| Roles | mature_transcript_region RNA |
| Sequences | BBa_K199039_sequence (Version 1) |
Description
Causes low expression of a suppressor tRNA that suppresses a 5-bp codon CCCUC, Part K199000.Notes
We used the pBad promoter to transcribe lower levels of tRNA in a high copy plasmid, pSB1A2.Source
The pBad promoter is from the BioBrick Parts registry. Suppressor tRNA was made by de novo synthesis from single-stranded oligos. Based on the paper by Anderson et al. 2002 (http://gcat.davidson.edu/GcatWiki/images/6/6f/AndersonSchultz2002ChemBiol.pdf).| Access | Instance | Definition |
| public public | BBa_I13453 BBa_K199000 | BBa_I13453 BBa_K199000 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_I13453 BBa_K199000 | 1,130 139,281 | BBa_I13453 BBa_K199000 |