BBa_K199079

BBa_K199079 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K199079

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Clifton Davis

Date created: 2009-09-19 11:00:00

Date modified: 2015-05-08 01:11:19

RBS RFP with CCACC Addition

Details

• Types: DnaRegion
• Roles: Reporter; engineered_region
• Sequences: BBa_K199079_sequence (Version 1)

Description

This RBS RFP reporter gene is a modification of BBa_E1010 with BBa_B0034; it has a 5-bp addition, CCACC, after the start codon and before the rest of the gene. This is used in conjunction with the CCACC tRNA(Part K199048). These CCACC tRNAs suppress the frameshift caused by this 5-bp addition in the reporter.

There is also a 25 bp addition after the double stop of the gene and before the Bio Brick Suffix. This addition is presumed to not effect the translation of the protein.

Notes

None

Source

The template of the gene comes from Part BBa_E1010. We designed a forward primer that included RBS,ATG, the 5-bp addition CCACC and the first 20 nucleotides on the 5' end of RFP after ATG. This made the edited 5' sequence for our RFP.

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
NcoI Restriction Site	442,442	sequence_feature, feature/misc
5-bp CCACC Addition	22,26	sequence_feature, feature/misc
25-bp Addition	705,729	feature/misc, sequence_feature

Other Properties

• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K199079/1

Member of these Collections

• iGEM Parts Registry