BBa_K1997004

BBa_K1997004 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K1997004

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Xinyuan Qiu

Date created: 2016-10-12 11:00:00

Date modified: 2016-10-16 10:23:08

sGFP-N->Zif268->sGFP-C

Details

• Types: DnaRegion
• Roles: Reporter; engineered_region
• Sequences: BBa_K1997004_sequence (Version 1)

Description

This is the N-terminal of split luciferase reporter. It can be used for protein-protein interaction researches.

Notes

This part is codon optimized for expression in E.coli This part is codon optimized for expression in E.coli, a 10aa linker was added to the both sides of the cds.

Source

This part is custom synthesized, the original sequence was acquired form fire fly

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
sGFP-N	1,471	CDS, feature/cds
10aa linker	472,501	CDS, feature/protein
zif268	514,774	feature/cds, CDS
10aa linker	787,816	feature/protein, CDS
sGFP-C	817,1062	feature/cds, CDS

Other Properties

• igem#experience: None
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K1997004/1

Member of these Collections

• iGEM Parts Registry