BBa_K1997013

BBa_K1997013 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K1997013

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Xinyuan Qiu

Date created: 2016-10-12 11:00:00

Date modified: 2016-10-13 06:38:51

P+R->sHRP-N->Zif-N->RBS->Zif-C->sHRP-C->TER

Details

• Types: DnaRegion
• Roles: Composite; engineered_region
• Sequences: BBa_K1997013_sequence (Version 1)

Description

This part is a integrated device for protein-protein interaction research using split-HRP system as a reporter

Notes

Two restriction sites for Gibson assembly was added at the flank of zinc-finger protein, so that such sequence could be sassily replaced.

Source

This part was synthesized by company

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_K1789012	1,223	engineered_region, feature/BioBrick
barcode	224,228	engineered_tag, feature/barcode
sHRP-N	229,870	CDS, feature/cds
10aa linker	871,900	feature/protein, CDS
Zif-N	913,1101	CDS, feature/cds
RBS	1102,1131	feature/rbs, ribosome_entry_site
Zif-C	1132,1209	CDS, feature/cds
10aa linker	1222,1251	feature/protein, CDS
sHRP-C	1252,1539	feature/cds, CDS
barcode	1540,1545	engineered_tag, feature/barcode
BBa_B0015	1546,1674	engineered_region, feature/BioBrick

Other Properties

• igem#experience: None
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K1997013/1

Member of these Collections

• iGEM Parts Registry