BBa_K1997020

BBa_K1997020 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K1997020
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Chushu Zhu
Date created: 2016-10-12 11:00:00
Date modified: 2016-10-13 07:39:21

P+R->GFP10->FRB->RBS->FKBP ->GFP11->RBS->GFP1-9-> TER



    • Details

    Types
    DnaRegion

    Roles
    Composite

    engineered_region

    Sequences BBa_K1997020_sequence (Version 1)

    Description

    This part is an integrated tool for protein-protein interaction research using split-GFP system as reporter.

    Notes

    We added BsaI sites at the flank of "FRB-RBS-FKBP" sequence so that this sequence can be easily replaced

    Source

    This part was synthesized by IDT

    Sequence Annotation Location Component / Role(s)
    BBa_K1789012
    barcode
    GFP10
    FRB
    RBS
    FKBP
    GFP11
    RBS
    GFP1-9
    barcode
    BBa_B0015
    		1,223
    224,228
    229,333
    346,630
    631,660
    661,984
    985,1107
    1108,1137
    1138,1728
    1729,1734
    1735,1863
    		engineered_region feature/BioBrick
    engineered_tag feature/barcode
    CDS feature/cds
    feature/cds CDS
    feature/rbs ribosome_entry_site
    CDS feature/cds
    CDS feature/cds
    feature/rbs ribosome_entry_site
    feature/cds CDS
    engineered_tag feature/barcode
    feature/BioBrick engineered_region

    igem#experience
    None
     
    igem#sampleStatus
    It's complicated
    igem#status
    Planning
     
    synbiohub#ownedBy
    user/james
     
    synbiohub#ownedBy
    user/myers
     
    synbiohub#topLevel
    BBa_K1997020/1
     

    Attachments

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