BBa_K1997021

BBa_K1997021 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K1997021
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Chushu Zhu
Date created: 2016-10-12 11:00:00
Date modified: 2016-10-13 07:41:16

P+R->sLuc-N->Zif268->sLuc-C->TER



Types
DnaRegion

Roles
Composite

engineered_region

Sequences BBa_K1997021_sequence (Version 1)

Description

This part is an integrated tool for protein-protein interaction research using split-LUC system as reporter. the "Zif268" subpart can be easily replaced using Golden Gate technique with BsaI

Notes

We added BsaI sites at the flank of "Zif268" sequence so that this sequence can be easily replaced

Source

This part was synthesized by IDT

Sequence Annotation Location Component / Role(s)
BBa_K1789012
barcode
sLUC-N
10aa linker
Zif268
10aa linker
sLUC-C
barcode
BBa_B0015
1,223
224,228
229,1476
1477,1506
1519,1779
1792,1821
1822,2280
2281,2286
2287,2415
feature/BioBrick engineered_region
feature/barcode engineered_tag
CDS feature/cds
CDS feature/protein
feature/cds CDS
CDS feature/protein
feature/cds CDS
feature/barcode engineered_tag
engineered_region feature/BioBrick
igem#experience
None
 
igem#sampleStatus
It's complicated
igem#status
Planning
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K1997021/1