BBa_K1998002

BBa_K1998002 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K1998002

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Shauna Winchester

Date created: 2016-10-11 11:00:00

Date modified: 2016-10-17 09:52:35

psbTB

Details

• Types: DnaRegion
• Roles: CDS; Coding
• Sequences: BBa_K1998002_sequence (Version 1)

Description

This part is composed of the psbT and psbB genes. The psbT protein's function is not well known however it appears to be involved in the dimerization of Photosystem II and is essential for photosynthetic activity.

Notes

Genes were codon optimised for bacteria using optimiser tool at http://genomes.urv.es/ OPTIMIZER/
Restriction sites were identified using Gene Designer, and removed by codon swapping, maintaining AA sequence.

Source

The genes were sourced from Chlamydomonas rienhardtii and synthesised

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
Plac	1,41	promoter, feature/promoter
psbT	60,164	feature/cds, CDS
RBS	43,56	ribosome_entry_site, feature/rbs
Start	57,59	feature/start, start_codon
psbB	165,1810	feature/cds, CDS

Other Properties

• igem#experience: None
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K1998002/1

Member of these Collections

• iGEM Parts Registry