Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Qin YE
Date created: 2016-10-09 11:00:00
Date modified: 2016-10-11 12:43:13
hKLF4-fl
| Types | DnaRegion |
| Roles | CDS Coding |
| Sequences | BBa_K2000006_sequence (Version 1) |
Description
hKLF4 (Human Kr??ppel-like factor4) is a member of the KLF family of transcription factors and regulates proliferation, differentiation, apoptosis and somatic cell reprogramming.we use this part to express full KLF4 gene in cancer cells.Notes
we find two illegal sites in this sequence and mutant them.Source
at gaggcagcca61 cctggcgagt ctgacatggc tgtcagcgac gcgctgctcc cgtccttctc cacgttcgcg
121 tccggcccgg cgggaaggga gaagacactg cgtccagcag gtgccccgaa taaccgctgg
181 cgggaggagc tctcccacat gaagcgactt cccccggtgc ttcccggccg cccctacgac
241 ctggcggcgg cgaccgtggc caccgacctg gagagtggag gagtcggcgc ggcttgcggc
301 agcagcaacc cggctcttct gccccggagg gagacggagg agttcaatga tctcctggac
361 ctggacttta tcctctccaa ctcgctgtcc catcaggagt ccgtggccgc cacggtgtcc
421 tcctcggcat cagcctcatc ctcgtcctcc ccgtcgagca gcggtccagc cagtgcgccc
481 tccacctgcc gc ttcagcta tccaatccgg gccgggggcg acccgggcgt ggcggcgccg
541 ggcggcgcgg gcggcggcct cctgtatggc cgggagtctg caccccctcc gacagctccc
601 ttcaacctgg cggacatcaa cgatgtgagc ccctccggcg gcttcgtggc cgagctcctg
661 cggcccgaat tggacccagt gtacattccg ccgcagcagc cgcagccgcc aggtggcggg
721 ctgatgggca agttcgtgtt gaaggcgtcg ctgagtgccc ctggcagcga gtacggcagc
781 ccgtcggtca tcagtgttag caaaggcagc ccagacggca gccacccggt ggtggtggcg
841 ccctacagcg gcgggccgcc acgcatgtgc ccgaagatca agcaggagac tgtctcctcg
901 tgcaccgtcg gtcggcccct agaggcccac ttgggcactg gacctcctct cagcaatggc
961 caccggccac ctgcgcacga ctttcccttg gggcggcagc tccccagcag gactaccccg
1021 accctgggtg ccgaggaact gctgagcagc cgggactgtc atcctgccct gccgctcccc
1081 ccgggcttcc atccccaccc cgggcccaac taccctccct tcctgcccga ccagatgcag
1141 ccgcaggtcc caccgctgca ttaccaagag ctcatgccac ccggttcctg catgccggag
1201 gagcccaaac caaagagggg aaggcggtcg tggccccgga aaaggacggc cactcacact
1261 tgtgattatg caggctgcgg caaaacctac acgaagagtt ctcatctcaa ggcacacctg
1321 cgcacccaca caggtgagaa accttaccac tgtgactggg atggctgtgg gtggaagttt
1381 gcccgctcag atgaactgac caggcactat cgcaaacaca ctgggcaccg ccccttccag
1441 tgccagaaat gcgaccgggc attctcgcgg tcggaccacc tcgccttaca tatgaaaagg
1501 cacttttaaa
| Sequence Annotation | Location | Component / Role(s) |
| KLF4 coding region | 1,1462 | feature/cds CDS |