BBa_K200001

BBa_K200001 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K200001
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Royah Vaezi, Dineka Khurmi
Date created: 2009-08-11 11:00:00
Date modified: 2015-05-08 01:11:21

Dam methylase -> Dam



Types
DnaRegion

Roles
Coding

CDS

Sequences BBa_K200001_sequence (Version 1)

Description

The methylase encoded by the dam gene (Dam methylase) transfers a methyl group from S-adenosylmethionine to the N6 position of the adenine residues in the sequence GATC. When methylation occurs in the recognition site of a particular group of restriction endonuclease including MboI, this protects the DNA from cleavage.

Notes

None

Source

This is one of three site-specific DNA methylases found in most laboratory strains of E. coli.

Sequence Annotation Location Component / Role(s)
Dam methylase gene
start
stop
Forward PCR primer
Reverse PCR primer
1,834
1,3
835,837
1,24
817,837
CDS feature/cds
start_codon feature/start
stop_codon feature/stop
feature/primer_binding primer_binding_site
primer_binding_site feature/primer_binding
igem#experience
None
 
igem#sampleStatus
It's complicated
igem#status
Planning
 
synbiohub#ownedBy
user/myers
 
synbiohub#ownedBy
user/james
 
synbiohub#topLevel
BBa_K200001/1