WaaL Ligas

BBa_K200003 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K200003

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Charles Fracchia, James Field

Date created: 2009-08-11 11:00:00

Date modified: 2015-05-08 01:11:21

Rfal

Details

• Types: DnaRegion
• Roles: Coding; CDS
• Sequences: BBa_K200003_sequence (Version 1)

Description

Unlike other exopolysaccharides, colanic acid does not naturally bind to the cell surface but rather forms a thick mesh between cells. While Waal Ligase usually links the O-antigen to the lipid A core, in K-12 it links colanic acid to the lipid A core. This is because E.coli K-12 does not produce O-antigen due to an insertion mutation.

Notes

N/A

Source

NCBI Reference Sequence: NC_000913.2
>gi|49175990:3794971-3796230 Escherichia coli str. K-12 substr. MG1655, complete genome

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
Waa Ligase	1,1257	CDS, feature/cds
start	1,3	start_codon, feature/start
stop	1258,1260	feature/stop, stop_codon
stop	1261,1263	feature/stop, stop_codon

Other Properties

• igem#experience: None
• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K200003/1

Member of these Collections

• iGEM Parts Registry