Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Rachelle Varga
Date created: 2016-10-16 11:00:00
Date modified: 2016-10-18 06:46:47
pVeg->sec-TD1 modular secretion platform with ThrC homology
| Types | DnaRegion |
| Roles | Device engineered_region |
| Sequences | BBa_K2008008_sequence (Version 1) |
Description
This part is meant to be used as a way to integrate any desired insert into the B. subtilis chromosome, which will then be secreted and possess the ability to diffuse transdermally through human skin.The integration cassette contains the constitutive B. subtilis promoter pVeg (BBa_K143012) and a strong B. subtilis RBS (BBa_K780001), as well as a B. subtilis secretory signal peptide sequence to allow for secretion of the desired protein product directly into surrounding media. A transdermal tag (TD1, BBa_K1074000) is included to allow for diffusion of the protein product across the skin.
The sequence contains two internal restriction enzyme sites: one for BamHI and one for XmaI. These sites allow for the directional cloning of a desired insert.
Note: This part is a composite part, not a basic part, but it could not be added as composite at the time of entry.
Notes
Addition of the AmyE homology regions are derived from B. subtilis 168 genomic DNA. Internal BamHI and XmaI restriction sites are present to allow for directional cloning of any desired insert. A secretory tag is present to allow for secretion of said insert, and a transdermal tag is present to allow the desired protein product to be secreted.Source
The pVeg promoter, sec secretion tag as well as the 125-bp AmyE regions of homology B. subtilis genome. TD1 is a transdermal tag first entered into the registry by the USTC_China iGEM team in 2013.| Sequence Annotation | Location | Component / Role(s) |
| 5 BBa_K143012 BBa_K780001 Sec tag BBa_K1074000 BamHI site XmaI site 3 | 1,67 68,164 165,180 190,276 277,309 313,318 325,330 337,461 | feature/dna sequence_feature promoter feature/promoter ribosome_entry_site feature/rbs feature/tag tag feature/tag tag feature/dna sequence_feature feature/dna sequence_feature sequence_feature feature/dna |