Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: William Cho
Date created: 2016-06-20 11:00:00
Date modified: 2016-10-08 01:21:43
ompT + sfGFP + PETase (PET-degrading enzyme, origin I. sakaiensis)
| Types | DnaRegion |
| Roles | Coding CDS |
| Sequences | BBa_K2010005_sequence (Version 1) |
Description
This His-tag is a sequence that allows a conjunct protein to be purified. The tag is composed of repetitions of histidines. This is an alternate version of BBa_K2010004, but serves the same function.Notes
The DNA sequence has been optimized for E. coli K12 and modified to facilitate gBlock synthesis, by avoiding repeated sequences.Source
This sequence is publicly known and was privately modified.| Sequence Annotation | Location | Component / Role(s) |
| T7 Promoter RBS 34 ompT Tag Linker sfGFP Linker PETase Linker His-Tag Stop Codon | 1,46 47,58 65,124 125,130 131,844 845,859 860,1729 1730,1765 1766,1783 1784,1789 | promoter feature/promoter ribosome_entry_site feature/rbs feature/tag tag sequence_feature feature/misc feature/protein CDS feature/misc sequence_feature CDS feature/protein sequence_feature feature/misc feature/tag tag stop_codon feature/stop |