Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Wenqi Huang
Date created: 2016-10-05 11:00:00
Date modified: 2016-11-01 09:59:16
Strong CheZ generator with a fusion GFP reporter
| Types | DnaRegion |
| Roles | Generator engineered_region |
| Sequences | BBa_K2012013_sequence (Version 1) |
Description
This generator contain promotor J23101 ,RBS from BBa_J61100,functionaal chemotaxis gene CheZ,reporter gene GFP from BBa_E0040 and a 18bp-GS linker between two functional domain.It can generate CheZ with a high ouIt is used to detect the relationship of CheZ under different promotor strength and its swimming ability.Notes
N/ASource
the CheZ coding sequence of this part is amplified from the wild type E.coli K12 MG1655 strain, the sequence is completely assistant with the sequence published on NCBI.| Sequence Annotation | Location | Component / Role(s) |
| BBa_J23101 BBa_J61100 CheZ BBa_E0040 18bp linker | 1,35 44,55 62,703 722,1438 704,721 | engineered_region feature/BioBrick feature/BioBrick engineered_region CDS feature/protein feature/BioBrick engineered_region sequence_feature feature/misc |