BBa_K2017003

BBa_K2017003 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K2017003
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Monica Victoria Gutierrez Salazar
Date created: 2016-10-02 11:00:00
Date modified: 2016-10-23 08:34:14

SAGTI linker + Luciferase



Types
DnaRegion

Roles
Reporter

engineered_region

Sequences BBa_K2017003_sequence (Version 1)

Description

Firefly luciferase protein. It oxidates its substrate luciferin, emitting light during the process. It can act as a reporter protein when used with a promoter. This part is made to be fused to the 3' with other coding sequence, so it includes the SAGTI linker in order to let the luciferase acquire the correct tertiary structure.

The luciferase does not include ATG (Met) to initiate translation, as it needs to be fused to 3' of other coding sequence. The translation must begin in the coding sequence fused to the 3'.
The linker includes a random nucleotide in 5', to change the reading frame of the luciferase. That way, it will not be translated unless an indel is produced in the coding region to which the part is fused.

Notes

This part must be fused to the 3' of other coding sequence. In order to be used in our modular gRNA testing system, it was necessary to remove the ATG (Met codon) to avoid unexpected translation. The translation must begin in the coding sequence fused to the 3'.
The linker includes a random nucleotide in 5', to change the reading frame of the luciferase. That way, it will not be translated unless an indel is produced in the coding region to which the part is fused.

Source

Luciferase was obtained from laboratory stock. The linker was fused with a PCR.

Sequence Annotation Location Component / Role(s)
Luciferase
SAGTI Linker
Additional nucleotide
16,1667
2,15
1,1
CDS feature/protein
feature/misc sequence_feature
feature/misc sequence_feature
igem#experience
Works
 
igem#status
Planning
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K2017003/1