Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: John Lazar
Date created: 2016-10-11 11:00:00
Date modified: 2016-10-15 12:46:47
mRFP + sgRNA targeting mRFP Coding Region (saCas9)
| Types | DnaRegion |
| Roles | Composite engineered_region |
| Sequences | BBa_K2019002_sequence (Version 1) |
Description
Part BBa_K2019002 consists of constitutively expressed mRFP gene (BBa_J04550) along with a constitutively expressed sgRNA that targets the mRFP gene.Notes
Considering that this construct would have to be cotransformed into a cell line with saCas9 expressed in the BBa_R0040-PSB1C3 backbone, all tests of this construct were in the PSB1T3 backbone.Source
The guide insert was designed using Benchling's gRNA design tool.| Sequence Annotation | Location | Component / Role(s) |
| BBa_J04450 BBa_J23119 mRFP Guide gRNA Scaffolding | 1,1068 1069,1104 1105,1125 1126,1207 | sequence_feature feature/dna promoter feature/promoter feature/dna sequence_feature sequence_feature feature/dna |