M5 TTL

BBa_K202000 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K202000

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Poonam Srivastava

Date created: 2009-10-15 11:00:00

Date modified: 2015-05-08 01:11:22

Hybrid promoter having multiple operator sites.

Details

• Types: DnaRegion
• Roles: promoter; Regulatory
• Sequences: BBa_K202000_sequence (Version 1)

Description

Hybrid promoter having multiple operator sites was designed according to the Lutz R and Bujard H.(Nucleic Acids Res. 1997 Mar 15;25(6):1203-10). The operator sequences are from three unrelated natural regulatory elements: the tetracycline (tet), lactose (lac) and λ-phage operons arranged logically within a single transcriptional unit. The regulatory architecture is designed such that each operator's position efficiently interferes with RNA polymerase (RNAp) promoter binding without inhibiting promoter function.
This promoter has tetO2 sites which are having transverse mutation at position 3.
The hybrid promoter is cloned upstream to the GFP (part Bba_E0240) in plasmid pSBA1. It is ready for assay.

Notes

two tetO2 sites and one lacO1 site was introduced in and around the Lambda phage.

Source

Synthetic construct having tet O2, lac O1 operator sites.

Other Properties

• igem#experience: Works
• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K202000/1

Member of these Collections

• iGEM Parts Registry