Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Nicola Freyer, Lea Steinbeck, Svenja Meyer, Alexander Deitert
Date created: 2016-10-03 11:00:00
Date modified: 2016-10-04 07:43:31
subtilisin E gene
| Types | DnaRegion |
| Roles | Other sequence_feature |
| Sequences | BBa_K2020000_sequence (Version 1) |
Description
This gene can be used to express subtilisin E in Escherichia coli. Subtilisin E is an alkaline serine protease which non-specifically deigests proteins. To use this part a suitable leader sequence has to be placed in front as the sequence of this BioBrick does not contain a start codon.Notes
The sequence from B. subtilis was codon optimized for E. coli with the DNA and protein sequence analysis software "Geneious" and additionally with the "Codon Optimization Tool" from IDT.Source
The sequence wasoriginally obtained from a wildtype Bacillus subtilis and was ordered from IDT.| Sequence Annotation | Location | Component / Role(s) |
| propeptide subtilisin E | 1,230 231,1059 | CDS feature/cds feature/cds CDS |